wyatt eclipse af4 system  (Waters Corporation)

Journal: Langmuir

Article Title: Dispersion Control of Fine Titanium Nitride Precipitates in Steels in the Liquid Phase Using the Hansen Solubility Parameter

doi: 10.1021/acs.langmuir.5c06212

Figure Lengend Snippet: AF4-ICP-MS analysis of TiN precipitates dispersed in (a) 1.73 mol m –3 SDS–methanol and (b) 1.73 mol m –3 SDS–EG/DMSO (50:50).

Article Snippet: For AF4-ICP-MS, a Wyatt Eclipse AF4 system (Wyatt Technology Europe, Germany) was combined with an Agilent 8800 ICP-MS system (Agilent Technologies, USA).

Techniques:

Journal: bioRxiv

Article Title: Structural heterogeneity in mRNA-LNP subpopulations revealed by AF4-SAXS: implications for cargo loading and cell transfection

doi: 10.64898/2026.01.16.699683

Figure Lengend Snippet: (a) DLS correlogram, (b) calculated size distribution from (a), and (c) SAXS data (markers) and model (line) acquired for LNPs without prior fractionation. The inset in (c) shows the single-phase dummy atom model of the scattering profile. (d) LNP size obtained by different ensemble-averaged methods: hydrodynamic radius ( R H ) from DLS, radius of gyration ( R g ) from SAXS, and optical radius ( R O ) from NanoFCM. (e) Cryo-EM micrograph highlighting the presence of different structures. Scale bar = 100 nm. (f) Schematic representation of the AF4 configuration and function. (g) AF4 fractograms showing the evolution of scattering-corrected UV absorbance at 280 nm, differential refractive index (dRI), and solvent-subtracted integrated SAXS intensity with elution time. The right Y-axis displays the changes in R g as determined from multi-angle light scattering (MALS) coupled to the AF4 setup. (h) SAXS profiles (markers) and models (lines) from different fractions (as indicated in the legend of the graph) and (i) dummy atom reconstructions of the scattering profiles highlighting the morphological variety. Data were collected for a sample with a pre-injection concentration of 4.25 mM total lipid and 300 µg/ml mRNA (N/P=3). SAXS data were fitted using the core-shell ellipsoid model combined with the Teubner-Strey equation. Error bars represent the standard deviations from the averaged values; where not seen, error bars are within the markers.

Article Snippet: The samples were fractionated on an AF4 system from Waters-Wyatt Technology Europe GmbH (Germany), equipped with an Eclipse Neon for flow control, with dilution control module (DCM) for split-outlet application, and a dispersion inlet (DI) channel for stop-less tip-injection frit-inlet application, with a 275 μm thick, wide (W) spacer, and a 10 kDa ultrafiltration membrane from regenerated cellulose acting as accumulation wall.

Techniques: Fractionation, Cryo-EM Sample Prep, Refractive Index, Solvent, Multi-Angle Light Scattering, Injection, Concentration Assay

Journal: bioRxiv

Article Title: Structural heterogeneity in mRNA-LNP subpopulations revealed by AF4-SAXS: implications for cargo loading and cell transfection

doi: 10.64898/2026.01.16.699683

Figure Lengend Snippet: AF4-SAXS data (markers) and fits (black lines) form model-based analysis for (a) N/P=3 and (b) N/P=6 nanoparticles. Data have been offset for clarity and elution time increases in the direction of the arrows. (c) and (d) show the p(r) profiles derived from IFT data analysis for the data shown in (a) and (b), respectively. AF4 fractograms showing the evolution of the normalized scattering-corrected UV absorbance at 280 nm and solvent-subtracted integrated SAXS intensity with elution time for (e) N/P=3 and (f) N/P=6. Shaded regions indicate the portions of the AF4 elution peak that were binned in 30 s intervals for subsequent SAXS data analysis. Main results from the structural characterization by AF4-SAXS: (g) particle concentration, (h) equatorial radius, (i) shell thickness, and (j) aspect ratio. (k) Parametric plot showing the correlation between particle sphericity and aspect ratio. Results from the analysis of cryo-EM micrographs: projected (l) particle area and (m) bleb area, defined as the area occupied by blebs within a particle. Particles analysis was performed using Fiji. Data were collected for samples with a pre-injection concentration of 4.25 mM total lipid and 150 µg/ml (N/P=6) or 300 µg/ml mRNA (N/P=3). Model-based analysis of SAXS data was performed using the core-shell ellipsoid model combined with the Teubner-Strey equation. Error bars represent the standard deviations from the averaged values; where not seen, error bars are within the markers. (n) Schematic representation of LNP structural evolution across the AF4 elution profile: early fractions contained compact spherical particles, intermediate fractions related slightly anisotropic prolate spheroids, and later fractions exhibited highly anisotropic, bleb-like assemblies.

Article Snippet: The samples were fractionated on an AF4 system from Waters-Wyatt Technology Europe GmbH (Germany), equipped with an Eclipse Neon for flow control, with dilution control module (DCM) for split-outlet application, and a dispersion inlet (DI) channel for stop-less tip-injection frit-inlet application, with a 275 μm thick, wide (W) spacer, and a 10 kDa ultrafiltration membrane from regenerated cellulose acting as accumulation wall.

Techniques: Derivative Assay, Solvent, Concentration Assay, Cryo-EM Sample Prep, Injection

Journal: bioRxiv

Article Title: Structural heterogeneity in mRNA-LNP subpopulations revealed by AF4-SAXS: implications for cargo loading and cell transfection

doi: 10.64898/2026.01.16.699683

Figure Lengend Snippet: AF4-SAXS data (markers) and models (solid lines) showing the evolution of the high q expansion of the data for particles formulated at N/P (a) 3 and (b) 6 at different elution times (as indicated in the legend of each graph). Micrographs of LNPs at N/P (c) 3 and (d) 6 focused on the structure of a representative particle. Scale bars = 50 nm. (e) Gray values extracted from the LNP micrographs shown in (c) and (d). Background gray value was 130±1. (f) Evolution of the scattering-corrected relative absorbance at 280 nm for N/P=3 and N/P=6. (g) Fraction of the particle core that is occupied by mRNA as determined from SAXS data analysis. (h) Amphiphile strength calculated from the results of the Teubner-Strey modelling. Results derived from NanoFCM analysis: (i) percentage of particles containing at least one mRNA copy and (j) average mRNA copy per particle. Particle electron density reconstructions from the SAXS data using DENSity from Solution Scattering (DENSS) for LNPs formulated at (k) N/P=3 and (l) N/P=6. The relative electron density shows how many standard deviations a region’s density differs from the average solvent background. Electron dense regions correspond to mRNA-rich and negative density corresponds to hydrophobic lipid regions. Error bars represent the standard deviations from the averaged values; where not seen, error bars are within the markers.

Article Snippet: The samples were fractionated on an AF4 system from Waters-Wyatt Technology Europe GmbH (Germany), equipped with an Eclipse Neon for flow control, with dilution control module (DCM) for split-outlet application, and a dispersion inlet (DI) channel for stop-less tip-injection frit-inlet application, with a 275 μm thick, wide (W) spacer, and a 10 kDa ultrafiltration membrane from regenerated cellulose acting as accumulation wall.

Techniques: Derivative Assay, Solvent

Journal: Current Research in Food Science

Article Title: Insights into the structure and retrogradation behavior of pullulanase modified potato starch using asymmetrical flow field-flow fractionation

doi: 10.1016/j.crfs.2025.101290

Figure Lengend Snippet: AF4-MALS-dRI fractograms, R g (a) and M w (b) distributions of PS and EPS treated with 45 U/g pullulanase at different pH values and 55 °C for 12 h.

Article Snippet: AF4 analysis of PS and EPS was performed using an Eclipse AF4 system (Wyatt, Dernbach, Germany) coupled with a DAWN HELEOS-II MALS detector (Wyatt, Santa Barbara, CA, USA) and a 1260 dRI detector (Agilent, Waldbronn, Germany), which was described in detail in our previous work ( ).

Techniques:

Journal: Current Research in Food Science

Article Title: Insights into the structure and retrogradation behavior of pullulanase modified potato starch using asymmetrical flow field-flow fractionation

doi: 10.1016/j.crfs.2025.101290

Figure Lengend Snippet: AF4-MALS-dRI fractograms, R g (a) and M w (b) distributions PS and EPS treated with 45 U/g pullulanase at 55 °C and pH 5.0 for different times.

Article Snippet: AF4 analysis of PS and EPS was performed using an Eclipse AF4 system (Wyatt, Dernbach, Germany) coupled with a DAWN HELEOS-II MALS detector (Wyatt, Santa Barbara, CA, USA) and a 1260 dRI detector (Agilent, Waldbronn, Germany), which was described in detail in our previous work ( ).

Techniques:

Journal: Current Research in Food Science

Article Title: Insights into the structure and retrogradation behavior of pullulanase modified potato starch using asymmetrical flow field-flow fractionation

doi: 10.1016/j.crfs.2025.101290

Figure Lengend Snippet: AF4-MALS-dRI fractograms, R g (a) and M w (b) distributions of PS and EPS treated with different enzyme contents at 55 °C and pH 5.0 for 12 h.

Article Snippet: AF4 analysis of PS and EPS was performed using an Eclipse AF4 system (Wyatt, Dernbach, Germany) coupled with a DAWN HELEOS-II MALS detector (Wyatt, Santa Barbara, CA, USA) and a 1260 dRI detector (Agilent, Waldbronn, Germany), which was described in detail in our previous work ( ).

Techniques:

Journal: Current Research in Food Science

Article Title: Insights into the structure and retrogradation behavior of pullulanase modified potato starch using asymmetrical flow field-flow fractionation

doi: 10.1016/j.crfs.2025.101290

Figure Lengend Snippet: AF4-MALS-dRI fractograms, R g (a) and M w (b) distributions of PS and EPS treated with 45 U/g pullulanase at different temperatures and pH 5.0 for 12 h.

Article Snippet: AF4 analysis of PS and EPS was performed using an Eclipse AF4 system (Wyatt, Dernbach, Germany) coupled with a DAWN HELEOS-II MALS detector (Wyatt, Santa Barbara, CA, USA) and a 1260 dRI detector (Agilent, Waldbronn, Germany), which was described in detail in our previous work ( ).

Techniques:

Journal: Current Research in Food Science

Article Title: Insights into the structure and retrogradation behavior of pullulanase modified potato starch using asymmetrical flow field-flow fractionation

doi: 10.1016/j.crfs.2025.101290

Figure Lengend Snippet: AF4-MALS-dRI fractograms, R g and M w distributions of PS (a and b) and EPS (c and d) stored at 4 °C for different times.

Article Snippet: AF4 analysis of PS and EPS was performed using an Eclipse AF4 system (Wyatt, Dernbach, Germany) coupled with a DAWN HELEOS-II MALS detector (Wyatt, Santa Barbara, CA, USA) and a 1260 dRI detector (Agilent, Waldbronn, Germany), which was described in detail in our previous work ( ).

Techniques: